School of Biological Sciences

Publication abstract

Isolation and characterization of microsatellite loci in the marine gastropod Nucella lapillus

Authors: Kawai, K., Hughes RN., Takenaka, O.,

Year of publication:2001

Journal: Molecular Ecology Notes, , Volume: 1(4), Pages: 270-272.

Publisher: Blackwell Science Ltd

Our paper deals the cloning and characterization of microsatellites from Nucella lapillus, and tests cross-species amplification in a congener and in two species of the confamilial genus Thais. Fourteen of 31 microsatellite loci tested were polymorphic, with 4-9 (mean 5.93) alleles per locus. The observed heterozygosity per locus varied from 0.10 to 0.85 (mean 0.37) and expected heterozygosity from 0.48 to 0.85 (mean 0.65). Most primer pairs were successfully amplified in N. freycineti, although only one primer pair was successfully amplified in both species of Thais. The markers are potentially useful for other species of Nucella.