Staff profile

Publications of Dr. Olga Golyshina

Journal publications

Golyshina OV, Toshchakov SV, Makarova KS, Gavrilov SN, Korzhenkov AA, La Cono V, Arcadi E, Nechitaylo TY, Ferrer M, Kublanov IV, Wolf YI, Yakimov MM, Golyshin PN. “ARMAN” archaea depend on association with euryarchaeal host in culture and in situ. Nat Commun.(2017) 8. doi:10.1038/s41467-017-00104-7.

Golyshina OV, Tran H, Reva ON, Lemak S, Yakunin AF, Alexander Goesmann A, Nechitaylo TY, LaCono V, Smedile F, Slesarev A, Rojo D, Barbas C, Ferrer M, Yakimov M and Golyshin PN. Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT. Sci Rep. (2017) Jun 16;7(1):3682. doi: 10.1038/s41598-017-03904-5.

Popovic A, Hai T, Tchigvintsev A, Hajighasemi M, Nocek B, Khusnutdinova AN, Brown G, Glinos J, Flick R, Skarina T, Chernikova TN, Yim V, Brüls T, Paslier DL, Yakimov MM, Joachimiak A, Ferrer M, Golyshina OV, Savchenko A, Golyshin PN,Yakunin AF. Activity screening of environmental metagenomic libraries reveals
novel carboxylesterase families. Sci Rep. (2017) Mar 8;7:44103. doi:10.1038/srep44103.

Golyshina OV, Kublanov IV, Tran H, Korzhenkov AA, Lünsdorf H, Nechitaylo TY,Gavrilov SN, Toshchakov SV, Golyshin PN. Biology of archaea from a novel family Cuniculiplasmataceae (Thermoplasmata) ubiquitous in hyperacidic environments. Sci Rep. (2016) Dec 14;6:39034. doi: 10.1038/srep39034.

Ferrer M, Martínez-Martínez M, Bargiela R, Streit WR, Golyshina OV, Golyshin PN. Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends. Microb Biotechnol. (2016) Jan;9(1):22-34. doi: 10.1111/1751-7915.12309.

Golyshina OV, Lünsdorf H, Kublanov IV, Goldenstein NI, Hinrichs KU, Golyshin PN. The novel extremely acidophilic, cell-wall-deficient archaeon Cuniculiplasma divulgatum gen. nov., sp. nov. represents a new family, Cuniculiplasmataceae fam. nov., of the order Thermoplasmatales. Int J Syst Evol Microbiol. (2016) Jan; 66(1):332-40. doi: 10.1099/ijsem.0.000725.

Ferrer M, Martínez-Martínez M, Bargiela R, Streit WR, Golyshina OV, Golyshin PN. Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends. Microb Biotechnol. (2016) Jan;9(1):22-34. doi: 10.1111/1751-7915.12309.

Popovic A, Tchigvintsev A, Tran H, Chernikova TN, Golyshina OV, Yakimov MM, Golyshin PN, Yakunin AF. Metagenomics as a Tool for Enzyme Discovery: Hydrolytic Enzymes from Marine-Related Metagenomes. Adv Exp Med Biol. (2015) 883:1-20. doi: 10.1007/978-3-319-23603-2_1. Review.

Placido A, Hai T, Ferrer M, Chernikova TN, Distaso M, Armstrong D, Yakunin AF, Toshchakov SV, Yakimov MM, Kublanov IV, Golyshina OV, Pesole G, Ceci LR, Golyshin PN. Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase. Appl Microbiol Biotechnol. (2015) Dec;99(23):10031-46. doi: 10.1007/s00253-015-6873-x.

Bargiela R, Mapelli F, Rojo D, Chouaia B, Tornés J, Borin S, Richter M, Del Pozo MV, Cappello S, Gertler C, Genovese M, Denaro R, Martínez-Martínez M, Fodelianakis S, Amer RA, Bigazzi D, Han X, Chen J, Chernikova TN, Golyshina OV, Mahjoubi M, Jaouanil A, Benzha F, Magagnini M, Hussein E, Al-Horani F, Cherif A,
Blaghen M, Abdel-Fattah YR, Kalogerakis N, Barbas C, Malkawi HI, Golyshin PN, Yakimov MM, Daffonchio D, Ferrer M. Bacterial population and biodegradation potential in chronically crude oil-contaminated marine sediments are strongly linked to temperature. Sci Rep. (2015) Jun 29;5:11651. doi: 10.1038/srep11651.

Méndez-García C, Peláez AI, Mesa V, Sánchez J, Golyshina OV, Ferrer M. Microbial diversity and metabolic networks in acid mine drainage habitats. Front Microbiol. (2015) May 29;6:475. doi: 10.3389/fmicb.2015.00475. eCollection 2015. Review. PubMed PMID: 26074887; PubMed Central PMCID: PMC4448039.

Castelle CJ, Roger M, Bauzan M, Brugna M, Lignon S, Nimtz M, Golyshina OV, Giudici-Orticoni MT, Guiral M. The aerobic respiratory chain of the acidophilic archaeon Ferroplasma acidiphilum: A membrane-bound complex oxidizing ferrous iron. Biochim Biophys Acta. (2015) Aug;1847(8):717-28. doi: 10.1016/j.bbabio.2015.04.006.

Alcaide M, Tchigvintsev A, Martínez-Martínez M, Popovic A, Reva ON, Lafraya Á, Bargiela R, Nechitaylo TY, Matesanz R, Cambon-Bonavita MA, Jebbar M, Yakimov MM, Savchenko A, Golyshina OV, Yakunin AF, Golyshin PN, Ferrer M; MAMBA Consortium. Identification and characterization of carboxyl esterases of gill
chamber-associated microbiota in the deep-sea shrimp Rimicaris exoculata by usingfunctional metagenomics. Appl Environ Microbiol. (2015) Mar;81(6):2125-36. doi: 10.1128/AEM.03387-14.

Privé F, Newbold CJ, Kaderbhai NN, Girdwood SG, Golyshina OV, Golyshin PN,
Scollan ND, Huws SA. Isolation and characterization of novel lipases/esterases from a bovine rumen metagenome. Appl Microbiol Biotechnol. (2015) Jul;99(13):5475-85. doi: 10.1007/s00253-014-6355-6.

Alcaide M, Stogios PJ, Lafraya Á, Tchigvintsev A, Flick R, Bargiela R, Chernikova TN, Reva ON, Hai T, Leggewie CC, Katzke N, La Cono V, Matesanz R, Jebbar M, Jaeger KE, Yakimov MM, Yakunin AF, Golyshin PN, Golyshina OV, Savchenko A, Ferrer M; MAMBA Consortium. Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats. Environ Microbiol. (2015) Feb;17(2):332-45. doi: 10.1111/1462-2920.12660.

Tchigvintsev A, Tran H, Popovic A, Kovacic F, Brown G, Flick R, Hajighasemi M, Egorova O, Somody JC, Tchigvintsev D, Khusnutdinova A, Chernikova TN, Golyshina OV, Yakimov MM, Savchenko A, Golyshin PN, Jaeger KE, Yakunin AF. The environment shapes microbial enzymes: five cold-active and salt-resistant
carboxylesterases from marine metagenomes. Appl Microbiol Biotechnol. (2015)
Mar;99(5):2165-78. doi: 10.1007/s00253-014-6038-3.

Jan C, Petersen JM, Werner J, Teeling H, Huang S, Glöckner FO, Golyshina OV, Dubilier N, Golyshin PN, Jebbar M, Cambon-Bonavita MA. The gill chamber epibiosis of deep-sea shrimp Rimicaris exoculata: an in-depth metagenomic investigation and discovery of Zetaproteobacteria. Environ Microbiol. (2014) Sep;16(9):2723-38. doi: 10.1111/1462-2920.12406.

Méndez-García C, Mesa V, Sprenger RR, Richter M, Diez MS, Solano J, Bargiela R, Golyshina OV, Manteca Á, Ramos JL, Gallego JR, Llorente I, Martins dos Santos VA, Jensen ON, Peláez AI, Sánchez J, Ferrer M. Microbial stratification in low pH oxic and suboxic macroscopic growths along an acid mine drainage. ISME J. (2014)
Jun;8(6):1259-74. doi: 10.1038/ismej.2013.242.

Werner J, Ferrer M, Michel G, Mann AJ, Huang S, Juarez S, Ciordia S, Albar JP, Alcaide M, La Cono V, Yakimov MM, Antunes A, Taborda M, da Costa MS, Hai T, Glöckner FO, Golyshina OV, Golyshin PN, Teeling H; MAMBA Consortium. Halorhabdus tiamatea: proteogenomics and glycosidase activity measurements identify the first cultivated euryarchaeon from a deep-sea anoxic brine lake as potential polysaccharide degrader. Environ Microbiol. (2014) Aug;16(8):2525-37. doi: 10.1111/1462-2920.12393

Yakimov MM, La Cono V, Slepak VZ, La Spada G, Arcadi E, Messina E, Borghini M, Monticelli LS, Rojo D, Barbas C, Golyshina OV, Ferrer M, Golyshin PN, Giuliano L. Microbial life in the Lake Medee, the largest deep-sea salt-saturated formation. Sci Rep. (2013) Dec 19;3:3554. doi: 10.1038/srep03554.

Kube M, Chernikova TN, Al-Ramahi Y, Beloqui A, Lopez-Cortez N, Guazzaroni ME, Heipieper HJ, Klages S, Kotsyurbenko OR, Langer I, Nechitaylo TY, Lünsdorf H, Fernández M, Juárez S, Ciordia S, Singer A, Kagan O, Egorova O, Petit PA, Stogios P, Kim Y, Tchigvintsev A, Flick R, Denaro R, Genovese M, Albar JP, Reva ON,
Martínez-Gomariz M, Tran H, Ferrer M, Savchenko A, Yakunin AF, Yakimov MM, Golyshina OV, Reinhardt R, Golyshin PN. Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. Nat Commun. (2013) 4:2156. doi: 10.1038/ncomms3156.

Alcaide M, Tornés J, Stogios PJ, Xu X, Gertler C, Di Leo R, Bargiela R, Lafraya A, Guazzaroni ME, López-Cortés N, Chernikova TN, Golyshina OV, Nechitaylo TY, Plumeier I, Pieper DH, Yakimov MM, Savchenko A, Golyshin PN, Ferrer M. Single residues dictate the co-evolution of dual esterases: MCP hydrolases from the α/β  hydrolase family. Biochem J. (2013) Aug 15;454(1):157-66. doi: 10.1042/BJ20130552.

Golyshin PN, Werner J, Chernikova TN, Tran H, Ferrer M, Yakimov MM, Teeling H, Golyshina OV; MAMBA Scientific Consortium. Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T). Genome Announc. (2013) Apr 18;1(2):e0014113. doi:
10.1128/genomeA.00141-13.

Del Pozo MV, Fernández-Arrojo L, Gil-Martínez J, Montesinos A, Chernikova TN, Nechitaylo TY, Waliszek A, Tortajada M, Rojas A, Huws SA, Golyshina OV, Newbold CJ, Polaina J, Ferrer M, Golyshin PN. Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail. Biotechnol Biofuels. (2012) Sep 21;5(1):73. doi:
10.1186/1754-6834-5-73.

Ferrer M, Ghazi A, Beloqui A, Vieites JM, López-Cortés N, Marín-Navarro J, Nechitaylo TY, Guazzaroni ME, Polaina J, Waliczek A, Chernikova TN, Reva ON, Golyshina OV, Golyshin PN. Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen. PLoS One. (2012) 7(6):e38134. doi: 10.1371/journal.pone.0038134.

Ferrer M, Werner J, Chernikova TN, Bargiela R, Fernández L, La Cono V, Waldmann J, Teeling H, Golyshina OV, Glöckner FO, Yakimov MM, Golyshin PN; MAMBA Scientific Consortium. Unveiling microbial life in the new deep-sea hypersaline Lake Thetis. Part II: a metagenomic study. Environ Microbiol. (2012) Jan;14(1):268-81. doi: 10.1111/j.1462-2920.2011.02634.x.

Navarro-Fernández J, Nechitaylo TY, Guerrero JA, Golyshina OV, García-Carmona F, Sánchez-Ferrer A, Golyshin PN. A novel platelet-activating factor acetylhydrolase discovered in a metagenome from the earthworm-associated microbial community. Environ Microbiol. (2011) Nov;13(11):3036-46. doi:10.1111/j.1462-2920.2011.02581.x.

Golyshina OV. Environmental, biogeographic, and biochemical patterns of archaea of the family Ferroplasmaceae. Appl Environ Microbiol. (2011) Aug;77(15):5071-8. doi: 10.1128/AEM.00726-11.

Vieites JM, Ghazi A, Beloqui A, Polaina J, Andreu JM, Golyshina OV, Nechitaylo TY, Waliczek A, Yakimov MM, Golyshin PN, Ferrer M. Inter-conversion of catalytic abilities in a bifunctional carboxyl/feruloyl-esterase from earthworm gut metagenome. Microb Biotechnol. (2010) Jan;3(1):48-58. doi: 10.1111/j.1751-7915.2009.00135.x.

Beloqui A, Polaina J, Vieites JM, Reyes-Duarte D, Torres R, Golyshina OV, Chernikova TN, Waliczek A, Aharoni A, Yakimov MM, Timmis KN, Golyshin PN, Ferrer M. Novel hybrid esterase-haloacid dehalogenase enzyme. Chembiochem. (2010) Sep 24;11(14):1975-8. doi: 10.1002/cbic.201000258.

Beloqui A, Nechitaylo TY, López-Cortés N, Ghazi A, Guazzaroni ME, Polaina J,
Strittmatter AW, Reva O, Waliczek A, Yakimov MM, Golyshina OV, Ferrer M, Golyshin PN. Diversity of glycosyl hydrolases from cellulose-depleting communities
enriched from casts of two earthworm species. Appl Environ Microbiol. (2010) Sep;76(17):5934-46. doi: 10.1128/AEM.00902-10.

Golyshina OV, Yakimov MM, Lünsdorf H, Ferrer M, Nimtz M, Timmis KN, Wray V, Tindall BJ, Golyshin PN. Acidiplasma aeolicum gen. nov., sp. nov., a euryarchaeon of the family Ferroplasmaceae isolated from a hydrothermal pool, and transfer of Ferroplasma cupricumulans to Acidiplasma cupricumulans comb. nov. Int J Syst Evol Microbiol. (2009) Nov;59(Pt 11):2815-23. doi: 10.1099/ijs.0.009639-0.

Ferrer M, Golyshina OV, Beloqui A, Böttger LH, Andreu JM, Polaina J, De LaceyAL, Trautwein AX, Timmis KN, Golyshin PN. A purple acidophilic di-ferric DNA ligase from Ferroplasma. Proc Natl Acad Sci U S A. (2008) Jul 1;105(26):8878-83. doi: 10.1073/pnas.0800071105.

López-Cortés N, Reyes-Duarte D, Beloqui A, Polaina J, Ghazi I, Golyshina OV, Ballesteros A, Golyshin PN, Ferrer M. Catalytic role of conserved HQGE motif inthe CE6 carbohydrate esterase family. FEBS Lett. (2007) Oct 2;581(24):4657-62. PubMed PMID: 17826771.

Ferrer M, Golyshina O, Beloqui A, Golyshin PN. Mining enzymes from extreme
environments. Curr Opin Microbiol. (2007) Jun;10(3):207-14. Review. PubMed PMID: 17548239.

Ferrer M, Beloqui A, Golyshina OV, Plou FJ, Neef A, Chernikova TN, Fernández-Arrojo L, Ghazi I, Ballesteros A, Elborough K, Timmis KN, Golyshin PN. Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome. Biotechnol J. (2007) Feb;2(2):207-13. PubMed PMID: 17238236.

Ferrer M, Golyshina OV, Beloqui A, Golyshin PN, Timmis KN. The cellular machinery of Ferroplasma acidiphilum is iron-protein-dominated. Nature. (2007) Jan 4;445(7123):91-4. PubMed PMID: 17203061.

Schneiker S, Martins dos Santos VA, Bartels D, Bekel T, Brecht M, Buhrmester J, Chernikova TN, Denaro R, Ferrer M, Gertler C, Goesmann A, Golyshina OV, Kaminski F, Khachane AN, Lang S, Linke B, McHardy AC, Meyer F, Nechitaylo T, Pühler A, Regenhardt D, Rupp O, Sabirova JS, Selbitschka W, Yakimov MM, Timmis KN, Vorhölter FJ, Weidner S, Kaiser O, Golyshin PN. Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. Nat Biotechnol. (2006) Aug;24(8):997-1004. Jul 30. PubMed PMID: 16878126.

Beloqui A, Pita M, Polaina J, Martínez-Arias A, Golyshina OV, Zumárraga M, Yakimov MM, García-Arellano H, Alcalde M, Fernández VM, Elborough K, Andreu JM, Ballesteros A, Plou FJ, Timmis KN, Ferrer M, Golyshin PN. Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships. J Biol Chem. (2006) Aug 11;281(32):22933-42. PubMed PMID: 16740638.

Golyshina OV, Golyshin PN, Timmis KN, Ferrer M. The 'pH optimum anomaly' of
intracellular enzymes of Ferroplasma acidiphilum. Environ Microbiol. (2006) Mar;8(3):416-25. PubMed PMID: 16478448.

Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Reyes-Duarte D, Santos VA, Strompl C, Elborough K, Jarvis G, Neef A, Yakimov MM, Timmis KN, Golyshin PN. Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora. Environ Microbiol. (2005) Dec;7(12):1996-2010. PubMed PMID: 16309396.

Ferrer M, Golyshina OV, Chernikova TN, Khachane AN, Martins Dos Santos VA, Yakimov MM, Timmis KN, Golyshin PN. Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin. Chem Biol. (2005) Aug;12(8):895-904. PubMed
PMID: 16125101.

Golyshina OV, Timmis KN. Ferroplasma and relatives, recently discovered cell wall-lacking archaea making a living in extremely acid, heavy metal-rich environments. Environ Microbiol. (2005) Sep;7(9):1277-88. Review. PubMed PMID: 16104851.

Ferrer M, Golyshina OV, Plou FJ, Timmis KN, Golyshin PN. A novel alpha-glucosidase from the acidophilic archaeon Ferroplasma acidiphilum strain Y with high transglycosylation activity and an unusual catalytic nucleophile. Biochem J. (2005) Oct 15;391(Pt 2):269-76. PubMed PMID: 15954864; PubMed Central PMCID: PMC1276924.

Golyshin PN, Martins Dos Santos VA, Kaiser O, Ferrer M, Sabirova YS, Lünsdorf  H, Chernikova TN, Golyshina OV, Yakimov MM, Pühler A, Timmis KN. Genome sequence completed of Alcanivorax borkumensis, a hydrocarbon-degrading bacterium that plays a global role in oil removal from marine systems. J Biotechnol. (2003) Dec 19;106(2-3):215-20. Review. PubMed PMID: 14651863.

Golyshina OV, Pivovarova TA, Karavaiko GI, Kondratéva TF, Moore ER, Abraham WR, Lünsdorf H, Timmis KN, Yakimov MM, Golyshin PN. Ferroplasma acidiphilum gen. nov., sp. nov., an acidophilic, autotrophic, ferrous-iron-oxidizing, cell-wall-lacking, mesophilic member of the Ferroplasmaceae fam. nov., comprising a distinct lineage of the Archaea. Int J Syst Evol Microbiol. (2000) May;50 Pt 3:997-1006. PubMed PMID: 10843038.

Karavaĭko GI, Golyshina OV, Troitskiĭ AV, Val'ekho-Roman KM, Golovacheva RS, Pivovarova TA. [Sulfurococcus yellowstonii sp. nov/--a new species of iron- and sulfur-oxidizing thermoacidophilic Archaeobacterium]. Mikrobiologiia. (1994) Jul-Aug;63(4):668-82. Russian. PubMed PMID: 7845250.

Karavaiko G.I., Smolskaja L.S., Golyshina O.V., Jagovkina M.A., Egorova E.Y. (1994) Bacterial pyrite oxidation: Influence of morphological, physical and chemical properties. Fuel Processing Technology 40: 151-165.

Golovacheva R.S., Golyshina O.V., Karavaiko G.I., Dorofeev A.G., Pivovarova T.A. Chernych N.A. (1992) A new iron-oxidizing bacterium, Leptospirillum thermoferrooxidans sp. nov. Microbiology 61: 744-750.

Soina V.S., Lebedeva E.V., Golyshina O.V., Fedorov-Davydov D.G.,  Gilichinsky D.A. (1991) Nitrifying bacteria from permafrost deposits of the Kolyma lowland. Microbiology 60: 187-190.

Book chapters:

Golyshina OV et al. Diversity and Physiologies of acidophilic Archaea. In Acidophiles. Life in extremely acidic environments. Ed. Quatrini R. and Johnson DB. Caister Academic Press. 2016. pp.93-107. DOI: https://doi.org/10.21775/9781910190333.06.

Golyshina OV et al. The family Ferroplasmaceae. In Bergey's Manual of Systematics of Archaea and Bacteria. Ed. John Wiley & Sons, Inc., in association with Bergey's Manual Trust. 2016. 1–3. DOI: 10.1002/9781118960608.fbm00273.

Golyshina OV et al. The genus Ferroplasma. In Bergey's Manual of Systematics of Archaea and Bacteria. Ed. John Wiley & Sons, Inc., in association with Bergey's Manual Trust. 2016. 1–6. DOI: 10.1002/9781118960608.gbm01373.

Golyshina OV et al. The genus Acidiplasma. In Bergey's Manual of Systematics of Archaea and Bacteria. Ed. John Wiley & Sons, Inc., in association with Bergey's Manual Trust. 2016. 1–7. DOI: 10.1002/9781118960608.gbm01372.

Golyshina OV. The family Ferroplasmaceae  In The Prokaryotes, 4th Edition(Rosenberg, DeLong,Thompson, Lory, Stackebrandt (Eds) Springer. 2014. pp.29-34.

Golyshin PN, Ferrer M, Chernikova TN, Golyshina OV, Yakimov MM. Oleispira. In The Handbook of hydrocarbon and lipid microbiology. In (Timmis (Ed) Springer. 2010. pp.1755-1763.

Cowan D.A., Kirby B.B., Meiring T.L., Ferrer M., Guazzaroni M.E., Golyshina O.V. and Golyshin P.N. 2010. Enzymes from Extreme Environments In: Manual of Industrial Microbiology and Biotechnology, 3rd Ed. (Baltz, Davies and Demain, eds.) ASM Press, Washington (DC) pp. 43-61. 

Patents:

  1. International Application WO 2006/00375 European Patent PCT/EP2005/053104. Cellulases from rumen (02.07.2004).
  2. International Application WO 2006/003009 European Patent PCT/EP2005/007305. Esterases from rumen. (06.07.2004).
  3. European Patent EP 05101628.5 Acidic enzymes from Ferroplasma (2005).
  4. European Patent. EP06003721.5 Polypeptides with laccase activity (2006).
  5. European Patent EP09003977.7 and US Patent US61/210,482. Probe compound for detecting and isolating enzymes and means and methods for using the same (2009). Licensed by Reactomix Ltd.