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Module FXX-3201:
Separation Science

Module Facts

Run by School of Natural Sciences

10.000 Credits or 5.000 ECTS Credits

Semester 1

Organiser: Dr Lorrie Murphy

Overall aims and purpose

The module addresses chromatography as the means to separate mixtures in synthetic and analytical chemistry. Chromatography is applied in different branches of chemistry in order to isolate sufficient quantities of a compound for further use or analytically to quantify a range of analytes using instrumental chromatography. The underlying principles of chromatography will be explained. The analytical process, as applied for the quantification of specific analytes in complex matrices will be explained. In addition, standard chromatography instruments will be detailed (HPLC-UV and GC-MS). The module aims to equip the student with the knowledge on separating mixtures and its application in synthetic and analytical chemistry.

Course content

The course addresses theory and principles of separation of mixtures using chromatography, including chromatography columns and application to the separation of reaction mixtures. Application for thin layer chromatography (TLC) and translation to preparative chromatography will be addressed in the interactive sessions. Focus will be on liquid chromatography (HLPC) with instrument design, column and mobile phases, separation strategies and different types of detectors. It will then address chromatography method descriptions as published in the scientific literature, manufacturers application notes and standard operating procedures. The application of chromatography in food, clinical or environmental analysis will be used to highlight the broad application range of this technique in industry, healthcare and public services. The module will also address instrumental chromatography, specifically gas chromatography (GC) in relation to instrumental design, stationary phases and parameters to improve separation and speed. Of particular importance are injectors and detectors, especially mass spectrometry (MS).

Assessment Criteria

excellent

Excellent (>70%). Knowledge base is extensive and extends well beyond the work covered in the programme. Conceptual understanding is outstanding. Problems of a familiar and unfamiliar nature are solved with efficiency and accuracy; problem-solving procedures are adjusted to the nature of the problem. Experimental work are exemplary and show a through analysis and appraisal of experimental results, with appropriate suggestions for improvement. Performance in transferable skills is generally very good.

threshold

Threshold (40%). Knowledge and understanding of the content covered in the course is basic; Problems of a routine nature are generally adequately solved; Standard laboratory experiments are usually carried out with reasonable success though significance and limitations of experimental data and/or observations may not be fully recognised; Transferable skills are at a basic level.

good

Good (~60%). Knowledge base covers all essential aspects of subject matter dealt with in the programme and shows good evidence of enquiry beyond this. Conceptual understanding is good. Problems of a familiar and unfamiliar nature are solved in a logical manner; solutions are generally correct and acceptable. Experimental work is carried out in a reliable and efficient manner, with a good appreciation of data analysis shown in write-ups. Performance in transferable skills is sound and shows no significant deficiencies.

Learning outcomes

  1. Students will be able to demonstrate that chromatography can be applied to different quantities of sample utilising different types of chromatography (TLC, column chromatography, HPLC, GLC) for qualitative and quantitative applications.

  2. Students will be able to demonstrate their understanding of the theory of chromatographic separation (resolution).

  3. Students will be able to compare and contrast the different professional information sources for analytical methods: Standard Operating Procedures, application notes, standard methods and scientific literature.

Assessment Methods

Type Name Description Weight
COURSEWORK Analysis of Journal/SOP/Application Note 50.00
CLASS TEST Separation Science Exam Online 50.00

Teaching and Learning Strategy

Hours
Lecture

9 x 2 hr Lecture/workshops

18
Private study

Private study

82

Transferable skills

  • Literacy - Proficiency in reading and writing through a variety of media
  • Numeracy - Proficiency in using numbers at appropriate levels of accuracy
  • Computer Literacy - Proficiency in using a varied range of computer software
  • Self-Management - Able to work unsupervised in an efficient, punctual and structured manner. To examine the outcomes of tasks and events, and judge levels of quality and importance
  • Exploring - Able to investigate, research and consider alternatives
  • Information retrieval - Able to access different and multiple sources of information
  • Critical analysis & Problem Solving - Able to deconstruct and analyse problems or complex situations. To find solutions to problems through analyses and exploration of all possibilities using appropriate methods, rescources and creativity.

Subject specific skills

  • CC4 The ability to recognise and analyse problems and plan strategies for their solution
  • CC6 Skills in communicating scientific material and arguments
  • CC8 The ability to adapt and apply methodology to the solution of unfamiliar problems
  • CP7 The ability to interpret and explain the limits of accuracy of their own experimental data in terms of significance and underlying theory
  • CP8 The ability to select appropriate techniques and procedures
  • CP11 The ability to use an understanding of the uncertainty of experimental data to inform the planning of future work
  • PS1 Communication skills, covering both written and oral communication with a variety of audiences
  • PS3 Problem-solving skills, relating to qualitative and quantitative information
  • PS7 Basic interpersonal skills, relating to the ability to interact with other people and to engage in teamworking
  • SK2 Demonstrate a systematic understanding of fundamental physicochemical principles with the ability to apply that knowledge to the solution of theoretical and practical problems
  • SK3 Gain knowledge of a range of inorganic and organic materials
  • SK5 Demonstrate an understanding of the qualitative and quantitative aspects of chemical metrology and the importance of traceability
  • SK9 Read and engage with scientific literature
  • CC1 the ability to demonstrate knowledge and understanding of essential facts,concepts,principles and theories relating to theSubject areasCovered in theirProgramme

Resources

Resource implications for students

I have suggested this book as an e-book to be held in the library.

Reading list

Chromatography: Basic Principles, Sample Preparations and Related Methods, authors Elsa Lundanes, Léon Reubsaet, Tyge Greibrokk, publisher Wiley

Courses including this module

Compulsory in courses:

  • F100: BSC Chemistry year 3 (BSC/C)
  • F102: Chem with Europ Exper year 4 (BSC/CEE)
  • F105: BSc Chemistry with International Experience year 4 (BSC/CHIE)
  • F103: BSC Chem with Ind Exper year 4 (BSC/CIE)
  • F104: MChem Chemistry year 3 (MCHEM/CH)
  • F106: MChem Chemistry with International Experience year 4 (MCHEM/CHIE)
  • F101: MChem Chemistry with Industrial Experience year 4 (MCHEM/CIND)

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