Professor Alexander Yakunin (Iakounine)

Office: Thoday Building, room S25

Email: a.iakounine@bangor.ac.uk

Tel: 01248 388399

Research focus: enzyme discovery, enzymology, enzyme applications

• PhD: Microbiology
  Winogradsky Institute of Microbiology, Moscow, 1987
• MSc: Molecular biology
  Moscow State University, 1978

2023

• CyhoeddwydCRISPR-induced DNA reorganization for multiplexed nucleic acid detection
  Karlikow, M., Amalfitano, E., Yang, X., Doucet, J., Chapman, A., Sadat-Moussavi, P., Homme, P., Sutyrina, P., Chang, W., Lemak, S., Yakunin, A., Dolezal, A. G., Kelley, S., Foster, L., Harpur, B. & Pardee, K., 17 Maw 2023, Yn: Nature Communications. 14, 1, 1505.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydHarnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling
  Williams, G., Ma, H., Khusnutdinova, A., Yakunin, A. & Golyshin, P., Awst 2023, Yn: Essays in Biochemistry. 67, 4, t. 715-729 15 t., EBC20220255.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl adolygu › adolygiad gan gymheiriaid
• E-gyhoeddi cyn argraffuStructural insights into hydrolytic defluorination of difluoroacetate by microbial fluoroacetate dehalogenases
  Khusnutdinova, A. N., Batyrova, K., Brown, G., Fedorchuck, T., Chai, Y. S., Skarina, T., Flick, R., Petit, A-P., Savchenko, A., Stogios, P. & Yakunin, A., 17 Gorff 2023, (E-gyhoeddi cyn argraffu) Yn: Febs Journal.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydThermophilic Carboxylesterases from Hydrothermal Vents of the Volcanic Island of Ischia Active on Synthetic and Biobased Polymers and Mycotoxins
  Distaso, M., Chernikova, T., Bargiela, R., Coscolín, C., Stogios, P. J., Gonzalez-Alfonso, J. L., Lemak, S., Khusnutdinova, A., Plou, F. J., Evdokimova, E., Savchenko, A., Lunev, E., Yakimov, M. M., Golyshina, O., Ferrer, M., Yakunin, A. & Golyshin, P., 28 Chwef 2023, Yn: Applied and Environmental Microbiology. 89, 2, t. e0170422 20 t.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2022

• CyhoeddwydMetabolite Damage and Damage-Control in a Minimal Genome
  Haas, D., Thamm, A. M., Sun, J., Huang, L., Sun, L., Beaudoin, G. A. W., Wise, K. S., Lema-Ortiz, C., Bruner, S. D., Breuer, M., Luthey-Schutten, Z., Lin, J., Wilson, M. A., Brown, G., Yakunin, A., Kurilyak, I., Folz, J., Fiehn, O., Glass, J. I., Hanson, A. D., Henry, C. S. & de Crecy-Lagard, V., 30 Awst 2022, Yn: mBio. e0163022.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2021

• CyhoeddwydStructural and biochemical insights into CRISPR RNA processing by the Cas5c ribonuclease SMU1763 from Streptococcus mutans
  Lemak, S., Serbanescu, M. A., Khusnutdinova, A. N., Ruszkowski, M., Beloglazova, N., Xu, X., Brown, G., Cui, H., Tan, K., Joachimiak, A., Cvitkovitch, D. G., Savchenko, A. & Yakunin, A., Tach 2021, Yn: Journal of Biological Chemistry. 297, 5, 101251.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2020

• CyhoeddwydOne-pot biocatalytic transformation of adipic acid to 6-aminocaproic acid and 1,6-hexamethylenediamine using carboxylic acid reductases and transaminases
  Fedorchuk, T. P., Khusnutdinova, A. N., Evdokimova, E., Flick, R., Di Leo, R., Stogios, P. J., Savchenko, A. & Yakunin, A., 15 Ion 2020, Yn: Journal of the American Chemical Society. 142, 2, t. 1038-1048 11 t.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydProteome cold-shock response in the extremely acidophilic archaeon, Cuniculiplasma divulgatum
  Bargiela, R., Lanthaler, K., Potter, C., Ferrer, M., Yakunin, A., Paizs, B., Golyshin, P. & Golyshina, O., 19 Mai 2020, Yn: Microorganisms . 8, 5
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2019

• CyhoeddwydPrenylated FMN: biosynthesis, purification, and Fdc1 activation
  Khusnutdinova, A. N., Xiao, J., Wang, P-H., Batyrova, K., Flick, R., Edwards, E. & Yakunin, A., 2019, New Approaches for Flavin Catalysis. Palfey, B. A. (gol.). Elsevier, Cyfrol 620. t. 469-488 (Methods in Enzymology).
  Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Pennod › adolygiad gan gymheiriaid
• CyhoeddwydSite-directed mutagenesis and stability of the carboxylic acid reductase MAB4714 from Mycobacterium abscessus
  Fedorchuk, T. P., Khusnutdinova, A. N., Flick, R. & Yakunin, A., 10 Medi 2019, Yn: Journal of Biotechnology. 303, t. 72-79
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2018

• CyhoeddwydBiosynthesis and activity of prenylated FMN cofactors
  Wang, P-H., Khusnutdinova, A. N., Luo, F., Xiao, J., Nemr, K., Flick, R., Brown, G., Mahadevan, R., Edwards, E. & Yakunin, A., 17 Mai 2018, Yn: Cell Chemical Biology. 25, 5, t. 560-570
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydDeterminants and prediction of esterase substrate promiscuity patterns
  Martínez-Martínez, M., Coscolín, C., Santiago, G., Chow, J., Stogios, P. J., Bargiela, R., Gertler, C., Navarro-Fernández, J., Bollinger, A., Thies, S., Méndez-García, C., Popovic, A., Brown, G., Chernikova, T., García-Moyano, A., Bjerga, G. E. K., Perez-Garcia, P., Hai, T., del Pozo, M. V., Stokke, R., Steen, I. H., Cui, H., Xu, X., Nocek, B., Alcaide, M., Disasto, M., Mesa, V., Pelaez, A. I., Sanchez, J., Buchholz, P. C. F., Pleiss, J., Fernández-Guerra, A., Glockner, F. O., Golyshina, O., Yakimov, M. M., Savchenko, A., Jaeger, K-E., Yakunin, A. F., Streit, W. R., Golyshin, P., Guallar, V. & Ferrer, M., 2018, Yn: ACS Chemical Biology. 13, 1, t. 225-234
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydEngineering a short, aldolase based pathway for (R)-1,3-butanediol production in Escherichia coli
  Nemr, K., Muller, J., Joo, J. C., Gawand, P., Cjoudhary, R., Mendonca, B., Lu, S., Yu, X., Yakunin, A. & Mahadevan, R., 1 Gorff 2018, Yn: Metabolic Engineering. 48, t. 13-24
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydHeavy metal removal by bioaccumulation using genetically engineered microorganisms
  Diep, P., Mahadevan, R. & Yakunin, A., 29 Hyd 2018, Yn: Frontiers in Bioengineering and Biotechnology. 6, 157.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydRelationships between Substrate Promiscuity and Chiral Selectivity of Esterases from Phylogenetically and Environmentally Diverse Microorganisms
  Coscolín, C., Martínez-Martínez, M., Chow, J., Bargiela, R., García-Moyano, A., Bjerga, G. E. K., Bollinger, A., Stokke, R., Steen, I. H., Golyshina, O., Yakimov, M. M., Jaeger, K-E., Yakunin, A. F., Streit, W. R., Golyshin, P. & Ferrer, M., 5 Ion 2018, Yn: Catalysts. 8, 1, 10.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydScreening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters
  Hajighasemi, M., Tchigvintsev, A., Nocek, B., Flick, R., Popovic, A., Hai, T., Khusnutdinova, A. N., Brown, G., Xu, X., Cui, H., Anstett, J., Chernikova, T., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Golyshina, O., Savchenko, A., Golyshin, P., Edwards, E. A. & Yakunin, A. F., 6 Tach 2018, Yn: Environmental Science and Technology. 52, 21, t. 12388-12401
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydStructural Insights into Substrate Selectivity and Activity of Bacterial Polyphosphate Kinases
  Nocek, B. P., Khusnutdinova, A. N., Ruszkowski, M., Flick, R., Burda, M., Batyrova, K., Brown, G., Mucha, A., Joachimiak, A., Berlicki, L. & Yakunin, A. F., 2 Tach 2018, Yn: ACS Catalysis. 8, 11, t. 10746-10760
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2017

• CyhoeddwydActivity screening of environmental metagenomic libraries reveals novel carboxylesterase families
  Popovic, A., Hai, T., Tchigvintsev, A., Hajighasemi, M., Nocek, B., Khusnutdinova, A. N., Brown, G., Glinos, J., Flick, R., Skarina, T., Chernikova, T., Yim, V., Bruls, T., Le Paslier, D., Yakimov, M. M., Joachimiak, A., Ferrer, M., Golyshina, O., Savchenko, A., Golyshin, P. & Yakunin, A. F., Maw 2017, Yn: Scientific Reports. 7, 44103, 44103.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydAlkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid
  Joo, J. C., Khusnutdinova, A. N., Flick, R., Kim, T., Bornscheuer, U. T., Yakunin, A. F. & Mahadevan, R., 1 Chwef 2017, Yn: Chemical Science. 8, 2, t. 1406-1413
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydMetabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT
  Golyshina, O., Hai, T., Reva, O. N., Lemak, S., Yakunin, A. F., Goesmann, A., Nechitaylo, T. Y., LaCono, V., Smedile, F., Slesarev, A., Rojo, D., Barbas, C., Ferrer, M., Yakimov, M. M. & Golyshin, P., 16 Meh 2017, Yn: Scientific Reports. 7, 3682.
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydSAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia
  Schneider, C., Oellerich, T., Baldauf, H-M., Schwarz, S-M., Thomas, D., Flick, R., Bohnenberger, H., Kaderali, L., Stegmann, L., Cremer, A., Martin, M., Lohmeyer, J., Michaelis, M., Hornung, V., Schliemann, C., Berdel, W. E., Hartmann, W., Wardelmann, E., Comoglio, F., Hansmann, M-L., Yakunin, A. F., Geisslinger, G., Stroebel, P., Ferreiros, N., Serve, H., Keppler, O. T. & Cinatl, J. J., Chwef 2017, Yn: Nature Medicine. 23, 2, t. 250-255
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2016

• CyhoeddwydA family of metal-dependent phosphatases implicated in metabolite damage-control
  Huang, L., Khusnutdinova, A., Nocek, B., Brown, G., Xu, X., Cui, H., Petit, P., Flick, R., Zallot, R., Balmant, K., Ziemak, M. J., Shanklin, J., de Crecy-Lagard, V., Fiehn, O., Gregory, J. F. . III., Joachimiak, A., Savchenko, A., Yakunin, A. F. & Hanson, A. D., Awst 2016, Yn: Nature Chemical Biology. 12, 8, t. 621-627
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydBiochemical and structural insights into enzymatic depolymerization of polylactic acid and other polyesters by microbial carboxylesterases
  Hajighasemi, M., Nocek, B. P., Tchigvintsev, A., Brown, G., Flick, R., Xu, X., Cui, H., Hai, T., Joachimiak, A., Golyshin, P., Savchenko, A., Edwards, E. A. & Yakunin, A. F., 18 Ebrill 2016, Yn: Biomacromolecules. 17, 6, t. 2027-2039
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2015

• CyhoeddwydCRISPR RNA binding and DNA target recognition by purified Cascade complexes from Escherichia coli
  Beloglazova, N., Kuznedelov, K., Flick, R., Datsenko, K. A., Brown, G., Popovic, A., Lemak, S., Semenova, E., Severinov, K. & Yakunin, A. F., Ion 2015, Yn: Nucleic Acids Research. 43, 1, t. 530-543
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydDiversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase
  Placido, A., Hai, T., Ferrer, M., Chernikova, T. N., Distaso, M. A., Armstrong, D., Yakunin, A. F., Toshchakov, S. V., Yakimov, M. M., Kublanov, I. V., Golyshina, O., Pesole, G., Ceci, L. R. & Golyshin, P., 1 Rhag 2015, Yn: Applied Microbiology and Biotechnology. 99, 23, t. 10031-10046
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydIdentification and characterization of carboxyl esterases of gill chamber-associated microbiota in the deep-sea shrimp Rimicaris exoculata using functional metagenomics
  Alcaide, M., Tchigvinstsev, A., Martinez-Martinez, M., Popovic, A., Reva, O. N., Lafraya, A., Bargiela, R. N., Nechitaylo, T. Y., Matesanz, R., Cambon-Bonavita, M., Jebbar, M., Savchenko, A., Yakimov, M. M., Golyshina, O., Yakunin, A. F., Golyshin, P., Ferrer, M. & The MAMBA Consortium., N. V., 16 Ion 2015, Yn: Applied and Environmental Microbiology. 81, 6, t. 2125-2136
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydMetagenomics as a tool for enzyme discovery: hydrolytic enzymes from marine-related metagenomes
  Popovic, A., Tchigvintsev, A., Tran, H., Chernikova, T. N., Golyshina, O. V., Yakimov, M. M., Golyshin, P. N. & Yakunin, A. F., 2015, Prokaryotic Systems Biology. Springer, t. 1-20 20 t.
  Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion Cynhadledd › Pennod › adolygiad gan gymheiriaid
• CyhoeddwydThe environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes
  Tchigvintsev, A., Tran, H., Popvic, A., Kovacic, F., Brown, G., Flick, R., Hajighasemi, M., Egorova, O., Somody, J. C., Tchigvintsev, D., Khusnutdinova, A., Chernikova, T. N., Golyshina, O., Yakimov, M. M., Savchenko, A., Golyshin, P., Jaeger, K. E. & Yakunin, A. F., 1 Maw 2015, Yn: Applied Microbiology and Biotechnology. 99, 5, t. 2165-2178
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2014

• CyhoeddwydPressure adaptation is linked to thermal adaptation in salt-saturated marine habitats
  Alcaide, M., Stogios, P. J., Lafraya, A., Tchigvintsev, A., Flick, R., Bargiela, R., Chernikova, T. N., Reva, O. N., Hai, T., Leggewie, C. C., Katzke, N., La Cono, V., Matesanz, R., Jebbar, M., Jaeger, K., Yakimov, M. M., Yakunin, A. F., Golyshin, P. N., Golyshina, O. V., Savchenko, A. & Ferrer, M., 17 Rhag 2014, Yn: Environmental Microbiology. 17, 2, t. 332-345
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
• CyhoeddwydThe CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity
  Lemak, S., Nocek, B., Beloglazova, N., Skarina, T., Flick, R., Brown, G., Joachimiak, A., Savchenko, A. & Yakunin, A. F., 29 Medi 2014, Yn: Nucleic Acids Research. 42, 17, t. 11144-11155
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2013

• CyhoeddwydGenome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
  Golyshina, O., Hai, T., Kube, M., Chernikova, T. N., Al-Ramahi, Y., Beloqui, A., Lopez-Cortez, N., Guazzaroni, M. E., Heipieper, H. J., Klages, S., Kotsyurbenko, O. R., Langer, I., Nechitaylo, T. Y., Lünsdorf, H., Fernández, M., Juárez, S., Ciordia, S., Singer, A., Kagan, O., Egorova, O., Petit, P. A., Stogios, P., Kim, Y., Tchigvintsev, A., Flick, R., Denaro, R., Genovese, M., Albar, J. P., Reva, O. N., Martínez-Gomariz, M., Tran, H., Ferrer, M., Savchenko, A., Yakunin, A. F., Yakimov, M. M., Golyshina, O. V., Reinhardt, R. & Golyshin, P. N., 23 Gorff 2013, Yn: Nature Communications. 4, t. 2156
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

2012

• CyhoeddwydStructure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica
  Lemak, S., Tchigvintsev, A., Petit, P., Flick, R., Singer, A. U., Brown, G., Evdokimova, E., Egorova, O., Gonzalez, C. F., Chernikova, T. N., Yakimov, M. M., Kube, M., Reinhardt, R., Golyshin, P. N., Savchenko, A. & Yakunin, A. F., 15 Gorff 2012, Yn: Biochemical Journal. 445, 2, t. 193-203
  Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

• 21ENGBIO: Polyphosphate-based synthetic pathway (PSP) for bioconversion of isopentenols to isoprenoid precursors using recombinant cell lysates

  31/01/2022 – 28/11/2023 (Wrthi'n gweithredu)

• Discovery of novel thermostable hydrolases from archaea of the Kuril-Kamchatka region

  01/12/2020 – 19/08/2023 (Wedi gorffen)