Module MSE-3035:
Laboratory Molecular Research
Laboratory Molecular Research 2023-24
MSE-3035
2023-24
School Of Medical And Health Sciences
Module - Semester 1
20 credits
Module Organiser:
Edgar Hartsuiker
Overview
The practical work will be carried out in a state of the art category 2 teaching/research laboratory. Tuition and experimental techniques are supported and enhanced via extensive use of audio/visual technology/tutorials and delivered by research active personal with extensive molecular experience and leaders of research groups focusing on medical-molecular research.
Practical work will employ the following techniques and methodologies
- Tissue culture growth and analysis of Human Cancer lines
- Extraction and purification of RNA from Human Cancer cells
- Fluorometric Quantitation, ‎Agarose gel and microfluidic chip analysis of RNA quality and quantity
- Reverse transcription cDNA synthesis
- Application of the Polymerase chain reaction (PCR) for RQ-PCR analysis of cDNA, RT-PCR molecular cloning, and recombinant plasmid detection (colony PCR).
- Plasmid extraction from bacteria
- Restriction enzyme digestion and ligation cloning of PCR fragments into plasmid vectors
- Methods of growth and selection of recombinant plasmid transformed bacteria
- Extensive utilisation of gel electrophoresis for DNA and RNA analysis and purification of specific DNA fragments
- Introduction to and application of bioinformatic DNA/RNA sequence analysis software
For student revision, lectures, tutorials and practical demonstrations are recorded using the Panopto system
Learning Outcomes
- Demonstrate abilities to prepare and present scientific reports and presentations, containing critical result analysis, to specifically designated formats, adhering to standards comparable to those used in academia and industry
- Demonstrate ability to critically process and analyse experimentally generated data
- Demonstrate broad knowledge and critical understanding of the theories and practical methods of nucleic acid isolation, purification, quantity and quality analysis
- Demonstrate broad knowledge and critical understanding of the theory and practical utilisation of restriction enzymes for molecular cloning
- Demonstrate broad knowledge and critical understanding of the theory and practical utilisation of the polymerase chain reaction (PCR) for molecular cloning and DNA analysis
- Demonstrate broad knowledge and critical understanding of transcription, reverse transcription and RNA transcripts
Assessment type
Crynodol
Description
Practical Report
Weighting
60%
Assessment type
Crynodol
Description
Practical Presentation
Weighting
40%