Module MSE-4042:
Laboratory Molecular Research

Module Facts

Run by School of Medical Sciences

20 Credits or 10 ECTS Credits

Semester 1

Organiser: Dr David Pryce

Overall aims and purpose

The aim of this module is to develop understanding of and provide substantial experience in the application of a number of key laboratory-based medical-molecular research methodologies. Over a two-week period, students will carry out a set of experiments developing extensive hands-on practical experience of laboratory-based medical-molecular research, to achieve an experimental research-led aim; the cloning of a human transcript into a bacterial plasmid vector

This module is available to International exchange students of The College of Health and Behavioural Sciences

Course content

The practical work will be carried out in a state of the art category 2 teaching/research laboratory. Tuition and experimental techniques are supported and enhanced via extensive use of audio/visual technology/tutorials and delivered by research active personal with extensive molecular experience and leaders of research groups focusing on medical-molecular research.

Practical work will employ the following techniques and methodologies

  • Tissue culture growth and analysis of Human Cancer lines
  • Extraction and purification of RNA from Human Cancer cells
  • Fluorometric Quantitation, ‎Agarose gel and microfluidic chip analysis of RNA quality and quantity
  • Reverse transcription cDNA synthesis
  • Application of the Polymerase chain reaction (PCR) for RQ-PCR analysis of cDNA, RT-PCR molecular cloning, and recombinant plasmid detection (colony PCR).
  • Plasmid extraction from bacteria
  • Restriction enzyme digestion and ligation cloning of PCR fragments into plasmid vectors
  • Methods of growth and selection of recombinant plasmid transformed bacteria
  • Extensive utilisation of gel electrophoresis for DNA and RNA analysis and purification of specific DNA fragments
  • Introduction to and application of bioinformatic DNA/RNA sequence analysis software

For student revision, lectures, tutorials and practical demonstrations are recorded using the Panopto system

Assessment Criteria

excellent

Distinction (A- to A**) (range 70-100%)

Primary criteria

Excellent students demonstrate comprehensive knowledge & detailed understanding of the subject area. Clear evidence of extensive background study & original thinking. Highly focussed answers and well structured. Arguments are logically presented and defended with evidence and examples. No factual/computational errors. Original interpretation of the information with clear evidence of wider reading. New links between topics are developed and new approaches to a problem are presented. Excellent presentation skills with very accurate communication.

Secondary Criteria

A* Outstanding

  • Exceeds expectations for most primary criteria
  • Complete command of subject and other relevant areas
  • Ideas/arguments are highly original

A+ Excellent

  • Exceeds expectations for some primary criteria
  • Complete command of subject
  • Ideas/arguments are highly original

A Good

  • Meets all primary criteria
  • Command of subject but with minor gaps in knowledge areas
  • Ideas/arguments are mostly original

A- Meets requirements of Class

  • Meets most but not all primary criteria
  • Complete command of subject but with some gaps in knowledge
  • Ideas/arguments are mostly original

good

Merit (B- to B+) (range 60-69%)

Primary criteria

Good students demonstrate strong knowledge & understanding of most but not all of the subject area. Limited evidence of background study. The answer is focussed with good structure. Arguments are presented coherently, mostly free of factual/computational errors. Some limited original interpretation. Well know links between topics are described. Problems are addressed by existing methods/approaches. Good presentation with accurate communication

Secondary Criteria

B+ Good

  • Exceeds expectations for most primary criteria
  • Command of subject but with gaps in knowledge
  • Some ideas/arguments original

B Mid-level

  • Meets all primary criteria
  • Strong factual knowledge and understanding
  • Ideas/arguments are well presented by few are original

B- Meets requirements of class

  • Meets most but not all primary criteria
  • Strong factual knowledge with minor weaknesses in understanding
  • Most but not all ideas/arguments are well presented and few are original

threshold

Pass (C- to C+) (range 50-59%)

Primary criteria

A threshold student demonstrates knowledge of key areas & principles, and understands the main elements of the subject area, although gaps and weaknesses in the argument are evident. No evidence of background study and wider reading. Answer focussed on question but also with some irrelevant material and weaknesses in structure & argument. Answers have several factual/computational errors. No original interpretation. No links between topics are described. Limited problem solving skills. Some weaknesses in presentation accuracy & delivery.

Secondary Criteria

C+ Good within the class

  • Exceeds expectations for some primary criteria
  • Strong factual knowledge with some weaknesses in understanding
  • Ideas/arguments are limited but are well presented

C Mid-level

  • Matches all primary criteria
  • Moderate factual knowledge with some weaknesses in understanding
  • Ideas/arguments are limited presented with weaknesses in logic/presentation

C- Meets requirements of class

  • Matches most but not all primary criteria
  • Moderate factual knowledge with several weaknesses in understanding
  • Ideas/arguments are limited presented with weaknesses in logic/presentation

Learning outcomes

  1. Demonstrate comprehensive knowledge and understanding of transcription, reverse transcription and RNA transcripts

  2. Demonstrate ability to fully process and critically analyse experimentally generated data

  3. Demonstrate comprehensive knowledge and understanding of the theory and practical utilisation of the polymerase chain reaction (PCR) for molecular cloning and DNA analysis

  4. Demonstrate master level abilities to prepare and present scientific reports and presentations, to specifically designated formats

  5. Demonstrate comprehensive knowledge and understanding of the theories and practical methods of nucleic acid isolation, purification, quantity and quality analysis

  6. Demonstrate comprehensive knowledge and understanding of the theory and practical utilisation of restriction enzymes for molecular cloning

Assessment Methods

Type Name Description Weight
REPORT Practical Report

The practical report will be written as a Research paper formatted for submission to the peer-reviewed scientific Journal Cell. It is estimated 30 hours of notional effort time is required in preparation for and undertaking of the report assessment

  • The Practical Report should be as concise as possible and written in a style that is accessible to the broad, scientific readership.
  • The abstract (summary) should be no more than 150 words (±10%), a Word count must be presented.
  • Highlights (4 maximum) and In Brief sections (of no more than 4 sentences).
  • The total word count for the rest of the report should be 3000 words, excluding bibliography/references. Word count must be presented at the beginning of the main report

Main Report should include

  • An introduction.
  • Results section, presenting a maximum of seven figures and/or data tables
  • Discussion section.
  • A maximum of 50 references can be included
  • Further guidelines available on "Results round up and write up discussion" on the module Evernote site
  • Practical report write up specifics can be discussed in tutorials.
  • Essential information will be recorded using the Panopto system.
60
INDIVIDUAL PRESENTATION Presentation

Students will give one individual presentation, on Wednesday, Thursday or Friday of week 16.

  • Each presentation assessment will take approximately 20 minutes, including at least 5 minutes allowance for questions. Feedback will then be provided
  • It is estimated 20 hours of notional effort time is required for preparation and undertaking the presentation assessment
  • Please note: To enable second marker assessment, student revision and feedback, all presentations will be individually Panopto recorded

The presentation and presenter should present:

  • One slide outlining the intended title for the practical report
  • One slide outlining and explaining the main overall aim of the practical and a maximum of 6 one sentence bullet pointed explanations of how the practical aim was to be achieved
  • A maximum of 3 slides presenting 'grouped' research methods/techniques employed to achieve the aim of a 'key section' of the practical. Each methods/techniques slide should have a clearly defined title highlighting the 'section aim'. Each slide should then present a concise description of the section aim and a logically ordered, bullet point lists of techniques utilized to achieve the aim
  • One key results figure slide, presenting one fully processed data figure. The figure must present what you consider to be the 'best data' for one 'key' result. The figure should be presented exactly as you intend it to presented in the final Practical Report. The data image must fully analyzed, include all essential annotations and have a full explanatory legend. You will be asked questions on this figure, in regards to the clarity of information presented, the data analysis and your understanding of the key results shown.
  • A final conclusions slide section presenting and explaining a set of the results 'highlights', to be included in the final report, and future possible work to potentially progress the final overall results.
40

Teaching and Learning Strategy

Hours
Tutorial

One tutorial at the end of the laboratory work. The tutorial will discuss class experimental results, with an interactive group review of analysis and report write up specifics; including overview of Journal format guidelines

3
Private study

Self-directed study to develop in-depth knowledge and understanding.

149
Laboratory

Two consecutive weeks of laboratory-based experiment practical work

  • First week: Monday, Tuesday, Thursday and Friday 9:30-5:00, Wednesday 9:30-1:00
  • Second week: Monday, Tuesday and Thursday 9:30-5:30, Wednesday 9:30-1:00

Results and analysis will be continually updated throughout as experimental stages are completed. Panopto recording will be employed as and when necessary.

48

Transferable skills

  • Literacy - Proficiency in reading and writing through a variety of media
  • Numeracy - Proficiency in using numbers at appropriate levels of accuracy
  • Self-Management - Able to work unsupervised in an efficient, punctual and structured manner. To examine the outcomes of tasks and events, and judge levels of quality and importance
  • Exploring - Able to investigate, research and consider alternatives
  • Information retrieval - Able to access different and multiple sources of information
  • Inter-personal - Able to question, actively listen, examine given answers and interact sensitevely with others
  • Critical analysis & Problem Solving - Able to deconstruct and analyse problems or complex situations. To find solutions to problems through analyses and exploration of all possibilities using appropriate methods, rescources and creativity.
  • Safety-Consciousness - Having an awareness of your immediate environment, and confidence in adhering to health and safety regulations
  • Teamwork - Able to constructively cooperate with others on a common task, and/or be part of a day-to-day working team
  • Argument - Able to put forward, debate and justify an opinion or a course of action, with an individual or in a wider group setting
  • Self-awareness & Reflectivity - Having an awareness of your own strengths, weaknesses, aims and objectives. Able to regularly review, evaluate and reflect upon the performance of yourself and others

Subject specific skills

Practical and theoretical skills

  • Ability to perform tissue culture growth and analysis of Human Cancer lines
  • Ability to extract and purify RNA from Human Cancer cell lines
  • Ability to perform Fluorometric Quantitation, ‎Agarose gel and microfluidic chip analysis of RNA quality and quantity
  • Ability to perform Reverse transcription cDNA synthesis
  • Ability to use and apply Polymerase chain reactions (PCR) in RQ-PCR analysis of cDNA, RT-PCR molecular cloning, and recombinant plasmid detection (colony PCR).
  • Ability to perform plasmid extraction from bacteria
  • Ability to perform Restriction enzyme digestion and ligation cloning of PCR fragments into plasmid vectors
  • Ability to grow and select recombinant plasmid transformed bacteria
  • Ability to perform agarose gel electrophoresis for DNA and RNA analysis and purification of specific DNA fragments
  • Ability to utilise bioinformatic DNA/RNA sequence analysis software for sequence verification and mutation detection

Resources

Resource implications for students

Access to a personal computer is beneficial, but not essential All resources needed to complete this module are provided. Computers can be accessed in multiple locations across the university. All teaching materials will be available on Blackboard. Completed assignments will be uploaded onto Turnitin through Blackboard. A limited number of the recommended textbooks can be found within the library.

Talis Reading list

http://readinglists.bangor.ac.uk/modules/mse-4042.html

Reading list

http://readinglists.bangor.ac.uk/modules/mse-4042.html

Pre- and Co-requisite Modules